First of all.. no need to be rude.
Seconds.. please check out the
XBMC wiki before posting next time.
And last.. here is the code:
CODE
import xbmc
# variable to contain the file location
file = "q:\\albums\\playlists\\startup.m3u"
# tell xbmc to play our file we specified in the above variable
xbmc.Player().play(file)